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recombinant human il 21 miltenyi biotec  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec recombinant human il 21 miltenyi biotec
    Recombinant Human Il 21 Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+il+21/Human+IL-21%2C+research+grade/pm42486103-554-23-26
    Average 94 stars, based on 12 article reviews
    recombinant human il 21 miltenyi biotec - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Activation Assay:

    Article Title: Anti-PD-L1 chimeric antigen receptor natural killer cell: Characterization and functional analysis.
    Article Snippet: Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences; Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz; Department of Immunology, School of Medicine, Dezful University of Medical Sciences, Dezful; Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences; Maternal-Fetal Medicine Research Center, Shiraz University of Medical Sciences; and Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

    Isolation:

    Article Title: Isolation of a tumor neoantigen specific CD8+ TCR from a skin biopsy of a vaccination site
    Article Snippet: .. To obtain APCs, autologous B cells were isolated by magnetic positive selection using CD19 MicroBeads (Miltenyi Biotec) and expanded with the Human CD40-Ligand Multimer Kit (Miltenyi Biotec) under manufacturer's recommendation in B cell media with 50UI/ml Human IL-4 (Miltenyi Biotec), 10UI/ml Human IL-21 (Miltenyi Biotec) and 30 ng/ml BAFF (Biolegend), hereafter, CD40-B cells. .. To test VILs reactivity CD40-B cells were pulsed ON with 10 μg/ml long peptides or short peptides and co-cultured the next day with VILs in filter plates with 0.45 um pore size Mixed Cellulose Esters membrane (Millipore), plating 100,000 B cells and 20,000 VILs or T cells per well in triplicates or duplicates, in screening or validation assays, respectively.

    Article Title: Isolation of a tumor neoantigen specific CD8+ TCR from a skin biopsy of a vaccination site
    Article Snippet: .. INF-gamma screening by ELISpot assay To obtain APCs, autologous B cells were isolated by magnetic positive selection using CD19 MicroBeads (Miltenyi Biotec) and expanded with the Human CD40-Ligand Multimer Kit (Miltenyi Biotec) under manufacturer's recommendation in B cell media with 50UI/ml Human IL-4 (Miltenyi Biotec), 10UI/ml Human IL-21 (Miltenyi Biotec) and 30 ng/ml BAFF (Biolegend), hereafter, CD40-B cells. .. To test VILs reactivity CD40-B cells were pulsed ON with 10 μg/ml long peptides or short peptides and co-cultured the next day with VILs in filter plates with 0.45 um pore size Mixed Cellulose Esters membrane (Millipore), plating 100,000 B cells and 20,000 VILs or T cells per well in triplicates or duplicates, in screening or validation assays, respectively.

    Selection:

    Article Title: Isolation of a tumor neoantigen specific CD8+ TCR from a skin biopsy of a vaccination site
    Article Snippet: .. To obtain APCs, autologous B cells were isolated by magnetic positive selection using CD19 MicroBeads (Miltenyi Biotec) and expanded with the Human CD40-Ligand Multimer Kit (Miltenyi Biotec) under manufacturer's recommendation in B cell media with 50UI/ml Human IL-4 (Miltenyi Biotec), 10UI/ml Human IL-21 (Miltenyi Biotec) and 30 ng/ml BAFF (Biolegend), hereafter, CD40-B cells. .. To test VILs reactivity CD40-B cells were pulsed ON with 10 μg/ml long peptides or short peptides and co-cultured the next day with VILs in filter plates with 0.45 um pore size Mixed Cellulose Esters membrane (Millipore), plating 100,000 B cells and 20,000 VILs or T cells per well in triplicates or duplicates, in screening or validation assays, respectively.

    Article Title: Isolation of a tumor neoantigen specific CD8+ TCR from a skin biopsy of a vaccination site
    Article Snippet: .. INF-gamma screening by ELISpot assay To obtain APCs, autologous B cells were isolated by magnetic positive selection using CD19 MicroBeads (Miltenyi Biotec) and expanded with the Human CD40-Ligand Multimer Kit (Miltenyi Biotec) under manufacturer's recommendation in B cell media with 50UI/ml Human IL-4 (Miltenyi Biotec), 10UI/ml Human IL-21 (Miltenyi Biotec) and 30 ng/ml BAFF (Biolegend), hereafter, CD40-B cells. .. To test VILs reactivity CD40-B cells were pulsed ON with 10 μg/ml long peptides or short peptides and co-cultured the next day with VILs in filter plates with 0.45 um pore size Mixed Cellulose Esters membrane (Millipore), plating 100,000 B cells and 20,000 VILs or T cells per well in triplicates or duplicates, in screening or validation assays, respectively.

    Control:

    Article Title: Universal allogeneic CAR T cells engineered with Sleeping Beauty transposons and CRISPR-CAS9 for cancer immunotherapy
    Article Snippet: .. Selective expansion of SB-CD19-28z.CAR/TCR KO T cells For the conventional selective expansion of SB-CD19-28z.CAR/TCR KO and SB-CD19-28.CAR/scrambled control T cells, the transfected cells were cocultured with 100 Gy-irradiated NALM6 feeder cells (TrueBeam STX radiotherapy system, Varian) at an effector-to-target ratio of 1:2 in the complete T cell medium in the presence of 50 U/mL human rIL-2 (Miltenyi Biotec), 30 ng/mL human IL-21 (Miltenyi Biotec), 10 ng/mL human IL-15 (Miltenyi Biotec), and 10 ng/mL human IL-7 (Miltenyi Biotec). ..

    Article Title: Supplemental Information Universal allogeneic CAR T cells engineered with Sleeping Beauty transposons and CRISPR-CAS9 for cancer immunotherapy
    Article Snippet: .. Selective expansion of SB-CD19-28z.CAR/TCR KO T cells For the conventional selective expansion of SB-CD19-28z.CAR/TCR KO and SB-CD19-28.CAR/scrambled control T cells, the transfected cells were cocultured with 100 Gy-irradiated NALM6 feeder cells (TrueBeam STX radiotherapy system, Varian) at an effector-to-target ratio of 1:2 in the complete T cell medium in the presence of 50 U/mL human rIL-2 (Miltenyi Biotec), 30 ng/mL human IL-21 (Miltenyi Biotec), 10 ng/mL human IL-15 (Miltenyi Biotec), and 10 ng/mL human IL-7 (Miltenyi Biotec). ..

    Transfection:

    Article Title: Universal allogeneic CAR T cells engineered with Sleeping Beauty transposons and CRISPR-CAS9 for cancer immunotherapy
    Article Snippet: .. Selective expansion of SB-CD19-28z.CAR/TCR KO T cells For the conventional selective expansion of SB-CD19-28z.CAR/TCR KO and SB-CD19-28.CAR/scrambled control T cells, the transfected cells were cocultured with 100 Gy-irradiated NALM6 feeder cells (TrueBeam STX radiotherapy system, Varian) at an effector-to-target ratio of 1:2 in the complete T cell medium in the presence of 50 U/mL human rIL-2 (Miltenyi Biotec), 30 ng/mL human IL-21 (Miltenyi Biotec), 10 ng/mL human IL-15 (Miltenyi Biotec), and 10 ng/mL human IL-7 (Miltenyi Biotec). ..

    Article Title: Supplemental Information Universal allogeneic CAR T cells engineered with Sleeping Beauty transposons and CRISPR-CAS9 for cancer immunotherapy
    Article Snippet: .. Selective expansion of SB-CD19-28z.CAR/TCR KO T cells For the conventional selective expansion of SB-CD19-28z.CAR/TCR KO and SB-CD19-28.CAR/scrambled control T cells, the transfected cells were cocultured with 100 Gy-irradiated NALM6 feeder cells (TrueBeam STX radiotherapy system, Varian) at an effector-to-target ratio of 1:2 in the complete T cell medium in the presence of 50 U/mL human rIL-2 (Miltenyi Biotec), 30 ng/mL human IL-21 (Miltenyi Biotec), 10 ng/mL human IL-15 (Miltenyi Biotec), and 10 ng/mL human IL-7 (Miltenyi Biotec). ..

    Magnetic Cell Separation:

    Article Title: Anti-PD-L1 chimeric antigen receptor natural killer cell: Characterization and functional analysis.
    Article Snippet: Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences; Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz; Department of Immunology, School of Medicine, Dezful University of Medical Sciences, Dezful; Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences; Maternal-Fetal Medicine Research Center, Shiraz University of Medical Sciences; and Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

    Enzyme-linked Immunospot:

    Article Title: Isolation of a tumor neoantigen specific CD8+ TCR from a skin biopsy of a vaccination site
    Article Snippet: .. INF-gamma screening by ELISpot assay To obtain APCs, autologous B cells were isolated by magnetic positive selection using CD19 MicroBeads (Miltenyi Biotec) and expanded with the Human CD40-Ligand Multimer Kit (Miltenyi Biotec) under manufacturer's recommendation in B cell media with 50UI/ml Human IL-4 (Miltenyi Biotec), 10UI/ml Human IL-21 (Miltenyi Biotec) and 30 ng/ml BAFF (Biolegend), hereafter, CD40-B cells. .. To test VILs reactivity CD40-B cells were pulsed ON with 10 μg/ml long peptides or short peptides and co-cultured the next day with VILs in filter plates with 0.45 um pore size Mixed Cellulose Esters membrane (Millipore), plating 100,000 B cells and 20,000 VILs or T cells per well in triplicates or duplicates, in screening or validation assays, respectively.



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